Coding

Part:BBa_K1590010:Design

Designed by: Manuel Blank   Group: iGEM15_Dundee   (2015-09-09)


Murine Spermine Binding Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix.

Primers for cloning Spermine Binding Protein into overexpression vector pQE80-L. 

  Forward: GCGC GGATCC CTGCTGCTGCTGACCCTGGCG

  Reverse: GCGC GGTACC TTAAGATTCGTCCTTCTGATC

Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3.


Source

In silico optimised version for E. coli K12 strains. Sequence ordered through IDT.

References